細菌培養基 L.B. Broth培養基 Lysogeny broth，LB (Miller配方)
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L.B. Broth培養基 Lysogeny broth，LB (Miller配方)
是微生物學實驗中最常用的營養性培養基(Miller配方)，用於培養大腸桿菌等細菌 如 DH5a，粉末狀培養基
產品
Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.
成份
Tryptone 10g/L
NaCl 10g/L
Yeast Extract 5g/L
應用
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
使用方法:
Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121oC to sterilize. Allow to cool before making additions, such as antibiotics (if desired)
是微生物學實驗中最常用的營養性培養基(Miller配方)，用於培養大腸桿菌等細菌 如 DH5a，粉末狀培養基
產品
Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.
成份
Tryptone 10g/L
NaCl 10g/L
Yeast Extract 5g/L
應用
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
使用方法:
Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121oC to sterilize. Allow to cool before making additions, such as antibiotics (if desired)
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